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Addgene

pFLAG-YAP1
(Plasmid #66853)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66853 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV FLAG
  • Backbone manufacturer
    Kodak (Sigma)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YAP1
  • Alt name
    Yes-associated protein 1
  • Species
    H. sapiens (human)
  • GenBank ID
    10413 NM_001130145.2
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

I subcloned the N-terminal PCR product (BamHI/XhoI) and the XhoI/KpnI fragment from pCMV SPORT YAP1 (Open biosytems) into BglII/KpnI sites of pCMV FLAG.
This is the original pCMV FLAG, with only one FLAG.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-YAP1 was a gift from Yutaka Hata (Addgene plasmid # 66853 ; http://n2t.net/addgene:66853 ; RRID:Addgene_66853)

  • For your References section:

    A cell-based assay to screen stimulators of the Hippo pathway reveals the inhibitory effect of dobutamine on the YAP-dependent gene transcription. Bao Y, Nakagawa K, Yang Z, Ikeda M, Withanage K, Ishigami-Yuasa M, Okuno Y, Hata S, Nishina H, Hata Y. J Biochem. 2011 Aug;150(2):199-208. doi: 10.1093/jb/mvr063. Epub 2011 May 17. 10.1093/jb/mvr063 PubMed 21586534
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