pAAV-Ef1a-DIO-OMOR-eYFP
(Plasmid #66847)

• Purpose: Expresses Opto-MOR in a Cre dependent manner
• Depositing Lab: Michael Bruchas
• Publication: Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 10.1016/j.neuron.2015.03.066. Epub 2015 Apr 30.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 6330
• Total vector size (bp): 7448
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Opto Mu opioid receptor
• Alt name: OMOR
• Alt name: Opto-MOR
• Species: R. norvegicus (rat)
• Insert Size (bp): 1118
• Promoter: Ef1a
• Tag / Fusion Protein:
  • eYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: gcttatcgataatcaacctctgg
• 3′ sequencing primer: GGT GAA CAG CTC CTC GCC CTT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry