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PurposeTet-inducible expression of GFP in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSLIK-hygro
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Backbone manufacturerIan Frasier, Addgene Plasmid #25737
- Backbone size w/o insert (bp) 13918
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP
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SpeciesAequorea victoria
- Promoter CMV
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer huB-Cpro-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLIK-GFP was a gift from Christian Metallo (Addgene plasmid # 66844 ; http://n2t.net/addgene:66844 ; RRID:Addgene_66844) -
For your References section:
Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells. Lewis CA, Parker SJ, Fiske BP, McCloskey D, Gui DY, Green CR, Vokes NI, Feist AM, Vander Heiden MG, Metallo CM. Mol Cell. 2014 Jul 17;55(2):253-63. doi: 10.1016/j.molcel.2014.05.008. Epub 2014 May 29. 10.1016/j.molcel.2014.05.008 PubMed 24882210