iRFP-PH-PLCdelta1
(Plasmid #66841)

• Purpose: expression of near-infrared PI(4,5)P2 biosensor
• Depositing Lab: Pietro De Camilli
• Publication: Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Modifications to backbone: iRFP (31857; Addgene) was amplified with flanking Nhe1/BsrG1 sites and used to replace GFP in pEGFP-C1.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PH-PLCdelta1
• Species: R. norvegicus (rat)
• Promoter: CMV
• Tag / Fusion Protein:
  • iRFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: Unknown primer sequence

Resource Information

• Supplemental Documents:
  • Plasmid Map from program ApE
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry