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Addgene

mCherry-CRY2-iSH2
(Plasmid #66839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66839 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iSH2
  • Alt name
    inter-SH2
  • Species
    H. sapiens (human)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry-CRY2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer Unknown primer sequence
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-CRY2-iSH2 was a gift from Pietro De Camilli (Addgene plasmid # 66839 ; http://n2t.net/addgene:66839 ; RRID:Addgene_66839)

  • For your References section:

    Optogenetic control of phosphoinositide metabolism. Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30. 10.1073/pnas.1211305109 PubMed 22847441
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