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PurposeExpression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name5ptaseOCRL
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Alt namephosphatidylinositol 5-phosphatase module of OCRL
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SpeciesH. sapiens (human)
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MutationD523G
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Entrez GeneOCRL (a.k.a. DENT2, Dent-2, INPP5F, LOCR, NPHL2, OCRL-1, OCRL1)
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry-CRY2 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PvuI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer Unknown primer sequence (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-CRY2-5ptaseOCRL(D523G) was a gift from Pietro De Camilli (Addgene plasmid # 66837 ; http://n2t.net/addgene:66837 ; RRID:Addgene_66837) -
For your References section:
Optogenetic control of phosphoinositide metabolism. Idevall-Hagren O, Dickson EJ, Hille B, Toomre DK, De Camilli P. Proc Natl Acad Sci U S A. 2012 Aug 28;109(35):E2316-23. doi: 10.1073/pnas.1211305109. Epub 2012 Jul 30. 10.1073/pnas.1211305109 PubMed 22847441