Myc-E-Syt3
(Plasmid
#66835)
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PurposeMammalian Expression, Bacterial Expression
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66835 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Modifications to backboneMyc epitope was synthesized in the pIDTSMART-Kan vector (Integrated DNA Technologies). Vector was digested with NheI and BamHI and the fragment was ligated into pEGFP-C1 to replace the EGFP with a Myc tag.
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameE-Syt3
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SpeciesH. sapiens (human)
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GenBank IDNM_031913
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Entrez GeneESYT3 (a.k.a. CHR3SYT, E-Syt3, FAM62C)
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Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer CCGCTCGAGCTatgcgagcagaggagccct
- 3′ sequencing primer ACCGGTCGACtcagcttctgggctgtccat (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myc-E-Syt3 was a gift from Pietro De Camilli (Addgene plasmid # 66835 ; http://n2t.net/addgene:66835 ; RRID:Addgene_66835) -
For your References section:
PI(4,5)P(2)-dependent and Ca(2+)-regulated ER-PM interactions mediated by the extended synaptotagmins. Giordano F, Saheki Y, Idevall-Hagren O, Colombo SF, Pirruccello M, Milosevic I, Gracheva EO, Bagriantsev SN, Borgese N, De Camilli P. Cell. 2013 Jun 20;153(7):1494-509. doi: 10.1016/j.cell.2013.05.026. 10.1016/j.cell.2013.05.026 PubMed 23791178