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Myc-E-Syt2
(Plasmid #66834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    Myc epitope was synthesized in the pIDTSMART-Kan vector (Integrated DNA Technologies). Vector was digested with NheI and BamHI and the fragment was ligated into pEGFP-C1 to replace the EGFP with a Myc tag.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E-Syt2
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_020728.1
  • Entrez Gene
    ESYT2 (a.k.a. CHR2SYT, E-Syt2, FAM62B)
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CAAGCTTCGAATTCgatgacgccaccgtcccgggc
  • 3′ sequencing primer GATCCGGTGGATCCctatgtcatcgcctgaggcctc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the E-syt2 coding region starts at the second methionine (M49) compared to the NCBI reference sequence; this shorter protein aligned more closely with endogenous E-Syt2 in SDS-PAGE. Both longer and shorter constructs displayed the same subcellular localization. Please see the associated publication for additional details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-E-Syt2 was a gift from Pietro De Camilli (Addgene plasmid # 66834 ; http://n2t.net/addgene:66834 ; RRID:Addgene_66834)

  • For your References section:

    PI(4,5)P(2)-dependent and Ca(2+)-regulated ER-PM interactions mediated by the extended synaptotagmins. Giordano F, Saheki Y, Idevall-Hagren O, Colombo SF, Pirruccello M, Milosevic I, Gracheva EO, Bagriantsev SN, Borgese N, De Camilli P. Cell. 2013 Jun 20;153(7):1494-509. doi: 10.1016/j.cell.2013.05.026. 10.1016/j.cell.2013.05.026 PubMed 23791178
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