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Addgene

pDD282 Notes

Addgene Diagnostic Digest- SpeI, ClaI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 66823 with the following enzymes Spe I and CIa I. Both enzymes should produce 7.3kb and 3.3kb bands. We see the expected results.

Addgene Diagnostic Digest- EcoRI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pDD282 with the following enzymes: EcoRI-HF.

Expected Bands
EcoRI-HF: ~10 kb

We observe the expected bands for this digest.