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pCCL-PGK-SPdCas9-BFP-DNMT3A
(Plasmid #66819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    3rd Generation lentiviral vector
  • Total vector size (bp) 12983
  • Modifications to backbone
    no
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dSpCas9-BFP-DNMT3A, catalytic domain of DNMT3A
  • Species
    Synthetic
  • Tag / Fusion Protein
    • dSpCas9-BFP-DNMT3A (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCCL-PGK-SPdCas9-BFP-DNMT3A was a gift from Yonglun Luo (Addgene plasmid # 66819 ; http://n2t.net/addgene:66819 ; RRID:Addgene_66819)

  • For your References section:

    Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases. Lin L, Liu Y, Xu F, Huang J, Daugaard TF, Petersen TS, Hansen B, Ye L, Zhou Q, Fang F, Yang L, Li S, Floe L, Jensen KT, Shrock E, Chen F, Yang H, Wang J, Liu X, Xu X, Bolund L, Nielsen AL, Luo Y. Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011. 10.1093/gigascience/giy011 PubMed 29635374
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