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Purpose(Empty Backbone) Shuttle vector with gram-positive origin of replication isolated from a native plasmid of Thermoanaerobacterium saccharolyticum B6A-RI
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Backbone manufacturerJoachim Messing lab
- Backbone size (bp) 6403
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMU131 was a gift from Lee Lynd (Addgene plasmid # 66811 ; http://n2t.net/addgene:66811 ; RRID:Addgene_66811) -
For your References section:
Natural competence in Thermoanaerobacter and Thermoanaerobacterium species. Shaw AJ, Hogsett DA, Lynd LR. Appl Environ Microbiol. 2010 Jul;76(14):4713-9. doi: 10.1128/AEM.00402-10. Epub 2010 May 14. 10.1128/AEM.00402-10 PubMed 20472726