pSLIK-IDH2-R172K-FLAG
(Plasmid #66807)

• Purpose: Tet-inducible expression of mutant IDH2 in mammalian cells
• Depositing Lab: Christian Metallo
• Publication: Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi: 10.1016/j.molcel.2014.05.008. Epub 2014 May 29.

Backbone

• Vector backbone: pSLIK-hygro
• Backbone manufacturer: Ian Frasier, Addgene Plasmid #25737
• Backbone size w/o insert (bp): 13918
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IDH2
• Species: H. sapiens (human)
• Insert Size (bp): 1471
• Mutation: R172K
• GenBank ID: NM_002168.3
• Entrez Gene: IDH2 (a.k.a. D2HGA2, ICD-M, IDH, IDH-2, IDHM, IDP, IDPM, mNADP-IDH)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: huB-Cpro-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert gene obtained from Patrick Ward and Craig Thompson in the form of pLPCX-IDH2-R172K

The depositor states that this plasmid behaves correctly based on on 2HG production (i.e. the metabolic activity of the enzyme).