pBlueScript NCX1.1
(Plasmid
#66799)
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 3210
- Total vector size (bp) 6120
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Vector typeT3 transcription of gene
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNCX1.1
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Alt nameSLC8A1
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SpeciesCanis lupus familiaris
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Insert Size (bp)2910
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Mutationthere are 3 silent mutations within the open reading frame
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GenBank IDNM_001304962.1
- Promoter T3
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3 Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBlueScript NCX1.1 was a gift from Michela Ottolia & Kenneth Philipson (Addgene plasmid # 66799 ; http://n2t.net/addgene:66799 ; RRID:Addgene_66799) -
For your References section:
Molecular cloning and functional expression of the cardiac sarcolemmal Na(+)-Ca2+ exchanger. Nicoll DA, Longoni S, Philipson KD. Science. 1990 Oct 26;250(4980):562-5. PubMed 1700476