pEIAV-DIO-TLoop-CHR2YFP
(Plasmid #66798)

• Purpose: expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector
• Depositing Lab: Edward Callaway
• Publication: Cetin et al J Neurophysiol. 2014 May;111(10):2150-9. doi: 10.1152/jn.00495.2013. Epub 2014 Feb 26.

Backbone

• Vector backbone: EIAV
• Backbone manufacturer: John Olsen UNC
• Backbone size w/o insert (bp): 4876
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Floxed Chr2-YFP
• Species: Chlamydomonas reinhardtii
• Insert Size (bp): 1655
• Mutation: H134R
• Promoter: CMVTetO7 Promoter
• Tag / Fusion Protein:
  • citrine (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: NheI (unknown if destroyed)
• 5′ sequencing primer: WPRE-R
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • pEIAV-DIO-TLoop-CHR2YFP
• A portion of this plasmid was derived from a plasmid made by: John Olsen, UNC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry