pAAV-cFos-tTA-pA
(Plasmid #66794)

• Purpose: Expresses TetActivator protein under the control of the c-fos promoter for use in TetTaging
• Depositing Lab: William Wisden
• Publication: Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi: 10.1038/nn.3957. Epub 2015 Feb 23.

Backbone

• Vector backbone: pUC
• Backbone size w/o insert (bp): 3600
• Total vector size (bp): 6673
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cfos promoter linked to the tet activator gene
• Species: Synthetic
• Insert Size (bp): 1945
• Tag / Fusion Protein:
  • no

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: GACCATCTCCGAAATCCTACACG
• 3′ sequencing primer: ACAAAGGCATTAAAGCAGCGTATCCACAT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cfos-tTA was taken from Addgene plasmid ID #: 34856
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry