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Addgene

pcDNA-Reb
(Plasmid #66792)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66792 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CG13183
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    CG13183 (a.k.a. Dmel_CG13183, Dmel\CG13183)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EcoRI
  • 3′ sequencing primer NotI
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To use this plasmid for S2 cell transfection, a short Kozac sequence was added 5' to the ATG of CG13183 : ttcAAG-ATG- GGA.... (GGA substitutes ATT from the original CG13183 sequence).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-Reb was a gift from Marta Llimargas Casanova (Addgene plasmid # 66792 ; http://n2t.net/addgene:66792 ; RRID:Addgene_66792)
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