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Addgene

pGEM-Teasy-Exp
(Plasmid #66791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T Easy
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3015
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CG13188
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1025
  • Mutation
    H452N (see depositor's comments below)
  • Entrez Gene
    CG13188 (a.k.a. Dmel_CG13188, Dmel\CG13188)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To use this plasmid for S2 cell transfection, a short Kozac sequence was added 5' to the ATG of CG13188 : ccGCG-ATG...

Mutation H452N does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-Teasy-Exp was a gift from Marta Llimargas Casanova (Addgene plasmid # 66791 ; http://n2t.net/addgene:66791 ; RRID:Addgene_66791)
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