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Addgene

pET28c-F30-Broccoli
(Plasmid #66788)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66788 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28c
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5215
  • Total vector size (bp) 5320
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Broccoli in the F30 scaffold
  • Species
    Synthetic
  • Insert Size (bp)
    105
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28c-F30-Broccoli was a gift from Samie Jaffrey (Addgene plasmid # 66788 ; http://n2t.net/addgene:66788 ; RRID:Addgene_66788)

  • For your References section:

    In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Filonov GS, Kam CW, Song W, Jaffrey SR. Chem Biol. 2015 May 21;22(5):649-60. doi: 10.1016/j.chembiol.2015.04.018. 10.1016/j.chembiol.2015.04.018 PubMed 26000751
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