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Addgene

NmCherry-SpVasa
(Plasmid #66786)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66786 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+8
  • Backbone manufacturer
    Addgene Plasmid #3493, Gökirmak et al. 2012, JBC
  • Backbone size w/o insert (bp) 4851
  • Vector type
    Mammalian Expression ; sea urchin, zebrafish, xenopus

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vasa
  • Species
    Strongylocentrotus purpuratus (Sea urchin)
  • Insert Size (bp)
    2301
  • Promoter SP6
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer TGTTGTTAACTTGTTTATTGCAGCT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NmCherry-SpVasa was a gift from Amro Hamdoun (Addgene plasmid # 66786 ; http://n2t.net/addgene:66786 ; RRID:Addgene_66786)

  • For your References section:

    Programmed reduction of ABC transporter activity in sea urchin germline progenitors. Campanale JP, Hamdoun A. Development. 2012 Feb;139(4):783-92. doi: 10.1242/dev.076752. 10.1242/dev.076752 PubMed 22274698
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