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pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]
(Plasmid #66781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR[1-2]
  • Backbone size w/o insert (bp) 2534
  • Total vector size (bp) 3045
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miniSOG
  • Species
    Synthetic
  • Insert Size (bp)
    511
  • Mutation
    G426C
  • Tag / Fusion Protein
    • tomm-20 (N terminal 55 aa) (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

426C of miniSOG supposedly cannot release singlet oxygen, see Shu et al, 2011 (PMID 21483721)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2] was a gift from Yishi Jin (Addgene plasmid # 66781 ; http://n2t.net/addgene:66781 ; RRID:Addgene_66781)

  • For your References section:

    Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG. Qi YB, Garren EJ, Shu X, Tsien RY, Jin Y. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7499-504. doi: 10.1073/pnas.1204096109. Epub 2012 Apr 24. 10.1073/pnas.1204096109 PubMed 22532663
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