pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]
(Plasmid
#66781)
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PurposeGly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOG
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66781 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepENTR[1-2]
- Backbone size w/o insert (bp) 2534
- Total vector size (bp) 3045
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameminiSOG
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SpeciesSynthetic
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Insert Size (bp)511
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MutationG426C
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Tag
/ Fusion Protein
- tomm-20 (N terminal 55 aa) (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
426C of miniSOG supposedly cannot release singlet oxygen, see Shu et al, 2011 (PMID 21483721)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2] was a gift from Yishi Jin (Addgene plasmid # 66781 ; http://n2t.net/addgene:66781 ; RRID:Addgene_66781) -
For your References section:
Photo-inducible cell ablation in Caenorhabditis elegans using the genetically encoded singlet oxygen generating protein miniSOG. Qi YB, Garren EJ, Shu X, Tsien RY, Jin Y. Proc Natl Acad Sci U S A. 2012 May 8;109(19):7499-504. doi: 10.1073/pnas.1204096109. Epub 2012 Apr 24. 10.1073/pnas.1204096109 PubMed 22532663
