-161+80pCalb2/dmutNRF-1(-41;-35)-like
(Plasmid #66741)

• Purpose: luciferase reporter for Calb2 promoter (-161to +80, mutant)
• Depositing Labs: Emanuela Felley-Bosco, Rolf Stahel
• Publication: Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/oncotarget.7114.

Backbone

• Vector backbone: pGL3 basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Calb2 promoter (-161bp+80)
• Alt name: calbindin 2
• Species: H. sapiens (human)
• Insert Size (bp): 241
• Mutation: CGCAGGCGC (-41;-35) to ATTAGGATT
• GenBank ID: 794
• Entrez Gene: CALB2 (a.k.a. CAB29, CAL2, CR)
• Promoter: Calb2
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: REVprimer3
• 3′ sequencing primer: GLprimer2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry