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Addgene

pBZSA FPR1 ECFP
(Plasmid #66725)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSSK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7560
  • Modifications to backbone
    Extensively modified
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Also resistant to Zeocin/Bleocin
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FPR1 (haplotype 3)-ECFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2060
  • GenBank ID
    M60627
  • Entrez Gene
    FPR1 (a.k.a. FMLP, FPR)
  • Promoter SR-alpha
  • Tag / Fusion Protein
    • ECFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer 5'-CTTCTAGGCCTGTACGG-3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ECFP was amplified from pECFP-C1 Clontech catalog #6076-1.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To create pBZSA, the neomycin-resistance gene was replaced with the zeocin-resistance gene (amplified as a SalI-SalI product from pZeo SV, Life Technologies.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBZSA FPR1 ECFP was a gift from Heini Miettinen (Addgene plasmid # 66725 ; http://n2t.net/addgene:66725 ; RRID:Addgene_66725)
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