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pBGSA FPR1 Q258A
(Plasmid #66724)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSSK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 8520
  • Modifications to backbone
    Extensively modified
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FPR1 (haplotype 3)
  • Alt name
    FMLP-R26
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1320
  • Mutation
    Q258A
  • GenBank ID
    M60627
  • Entrez Gene
    FPR1 (a.k.a. FMLP, FPR)
  • Promoter SR-alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5'-CTTCTAGGCCTGTACGG-3'
  • 3′ sequencing primer 5'-TCTAGAACTAGTGGATCC-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This mutant shows constitutive chemotaxis and beta-arrestin binding. Also associated with PMID: 16038804.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBGSA FPR1 Q258A was a gift from Heini Miettinen (Addgene plasmid # 66724 ; http://n2t.net/addgene:66724 ; RRID:Addgene_66724)

  • For your References section:

    The ligand binding site of the formyl peptide receptor maps in the transmembrane region. Miettinen HM, Mills JS, Gripentrog JM, Dratz EA, Granger BL, Jesaitis AJ. J Immunol. 1997 Oct 15;159(8):4045-54. PubMed 9378994
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