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pBGSA FPR1 N297A
(Plasmid #66723)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66723 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSSK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 8520
  • Modifications to backbone
    Extensively modified
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FPR1 (haplotype 3)
  • Alt name
    FMLP-R26
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1320
  • Mutation
    N297A
  • GenBank ID
    M60627
  • Entrez Gene
    FPR1 (a.k.a. FMLP, FPR)
  • Promoter SR-alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer 5'-CTTCTAGGCCTGTACGG-3'
  • 3′ sequencing primer 5'-TCTAGAACTAGTGGATCC-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This mutant is defective in phosphorylation and beta-arrestin binding which leads to a defect in desensitization. Also associated with PMID: 11085933.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBGSA FPR1 N297A was a gift from Heini Miettinen (Addgene plasmid # 66723 ; http://n2t.net/addgene:66723 ; RRID:Addgene_66723)

  • For your References section:

    Formyl peptide receptor-mediated ERK1/2 activation occurs through G(i) and is not dependent on beta-arrestin1/2. Gripentrog JM, Miettinen HM. Cell Signal. 2008 Feb;20(2):424-31. Epub 2007 Nov 9. 10.1016/j.cellsig.2007.11.002 PubMed 18060741
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