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PurposeExpresses NleL (170-782) in E. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66716 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMCSG17
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNleL
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Entrez GeneNEWENTRY
- Promoter T7
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Tag
/ Fusion Protein
- GST-TEV (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCSG17-NleL was a gift from David Komander (Addgene plasmid # 66716 ; http://n2t.net/addgene:66716 ; RRID:Addgene_66716) -
For your References section:
Assembly, analysis and architecture of atypical ubiquitin chains. Hospenthal MK, Freund SM, Komander D. Nat Struct Mol Biol. 2013 May;20(5):555-65. doi: 10.1038/nsmb.2547. Epub 2013 Apr 7. 10.1038/nsmb.2547 PubMed 23563141