pOPINK-UBE2S
(Plasmid
#66714)
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PurposeExpresses UBE2S (1-196) in E. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOPINK
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Backbone manufacturerOPPF-UK
- Backbone size w/o insert (bp) 6299
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namee2-epf
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Entrez GeneUBE2S (a.k.a. E2-EPF, E2EPF, EPF5)
- Promoter T7
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Tag
/ Fusion Protein
- GST-3C (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINK-UBE2S was a gift from David Komander (Addgene plasmid # 66714 ; http://n2t.net/addgene:66714 ; RRID:Addgene_66714) -
For your References section:
Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne. Bremm A, Freund SM, Komander D. Nat Struct Mol Biol. 2010 Aug;17(8):939-47. doi: 10.1038/nsmb.1873. Epub 2010 Jul 11. 10.1038/nsmb.1873 PubMed 20622874