pGEX6P1-UBE2S-UBD
(Plasmid #66713)

• Purpose: Expresses the UBE2S-UBD fusion protein in E. coli
• Depositing Lab: David Komander
• Publication: Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-47. doi: 10.1038/nsmb.1873. Epub 2010 Jul 11.

Backbone

• Vector backbone: pGEX6P1
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4984
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: e2-epf
• Entrez Gene: UBE2S (a.k.a. E2-EPF, E2EPF, EPF5)
• Promoter: T7
• Tag / Fusion Protein:
  • GST-3C (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Gene/Insert 2

• Gene/Insert name: isot
• Entrez Gene: USP5 (a.k.a. ISOT)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry