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PurposeExpresses activated AMSH in E. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOPINB
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Backbone manufacturerOPPF-UK
- Backbone size w/o insert (bp) 5531
- Total vector size (bp) 6713
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert namehbp
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SpeciesM. musculus (mouse)
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Entrez GeneStam2 (a.k.a. 1200004O12Rik, 5730456G07Rik, BB145015, Hbp)
- Promoter T7
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Tag
/ Fusion Protein
- His-3C (N terminal on backbone)
Cloning Information for Gene/Insert 1
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameAMSH
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SpeciesH. sapiens (human)
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Entrez GeneSTAMBP (a.k.a. AMSH, MICCAP)
- Promoter T7
Cloning Information for Gene/Insert 2
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINB-AMSH* was a gift from David Komander (Addgene plasmid # 66712 ; http://n2t.net/addgene:66712 ; RRID:Addgene_66712) -
For your References section:
Assembly and specific recognition of k29- and k33-linked polyubiquitin. Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D. Mol Cell. 2015 Apr 2;58(1):95-109. doi: 10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5. 10.1016/j.molcel.2015.01.042 PubMed 25752577