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PurposeExpresses AREL1 HECT domain in E. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOPINS
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Backbone manufacturerOPPF-UK
- Backbone size w/o insert (bp) 5906
- Total vector size (bp) 7076
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameKIAA0317
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Entrez GeneAREL1 (a.k.a. KIAA0317)
- Promoter T7
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Tag
/ Fusion Protein
- His-SUMO (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINS-AREL1 was a gift from David Komander (Addgene plasmid # 66710 ; http://n2t.net/addgene:66710 ; RRID:Addgene_66710) -
For your References section:
Assembly and specific recognition of k29- and k33-linked polyubiquitin. Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D. Mol Cell. 2015 Apr 2;58(1):95-109. doi: 10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5. 10.1016/j.molcel.2015.01.042 PubMed 25752577