pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
(Plasmid
#66708)
-
Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneFUGW
- Total vector size (bp) 15000
-
Vector typeLentiviral, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Stbl3
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameS.Pyogenes VP64-dCas9-VP64
-
SpeciesS.Pyogenes
-
MutationD10A and H840A
- Promoter hUbC
-
Tag
/ Fusion Protein
- VP64
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TCGGGTTTATTACAGGGACAGCAG
- 3′ sequencing primer TGACAACGGGCCACAACTCC (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites) was a gift from Charles Gersbach (Addgene plasmid # 66708 ; http://n2t.net/addgene:66708 ; RRID:Addgene_66708)
Map uploaded by the depositor.
