pCX-Klf2
(Plasmid
#66655)
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PurposeFor expression of mouse Klf2.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCX
- Total vector size (bp) 5858
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKlf2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1065
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Entrez GeneKlf2 (a.k.a. Lklf)
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer tttgtcccaaatctgtgcgg
- 3′ sequencing primer gctcaaggggcttcatgatg (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byKlf2 came from plasmid pMXs-ms-Klf2 (Addgene plasmid #50786).
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The backbone of the plasmid is pCX-OKS-2A (Addgene plasmid #19771).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCX-Klf2 was a gift from Barak Cohen (Addgene plasmid # 66655 ; http://n2t.net/addgene:66655 ; RRID:Addgene_66655) -
For your References section:
Interactions between pluripotency factors specify cis-regulation in embryonic stem cells. Fiore C, Cohen BA. Genome Res. 2016 Jun;26(6):778-86. doi: 10.1101/gr.200733.115. Epub 2016 Apr 15. 10.1101/gr.200733.115 PubMed 27197208