pGL-Pou
(Plasmid #66654)

• Purpose: Parent vector used for inserting cis-regulatory elements (CREs) for CRE-seq in ES cells. Uses the Pou5f1 basal promoter and dsRed gene.
• Depositing Lab: Barak Cohen
• Publication: Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi: 10.1101/gr.200733.115. Epub 2016 Apr 15.

Backbone

• Vector backbone: pGL4
• Backbone manufacturer: Promega
• Total vector size (bp): 3668
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dsRed-Express2
• Species: Synthetic
• Insert Size (bp): 674
• Promoter: Mouse Pou5f1 basal promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: TCATTACATCTGTGTGTTGGTTTTT
• 3′ sequencing primer: CCCCCTGAACCTGAAACATA

Resource Information

• Supplemental Documents:
  • Ape file of annotated plasmid sequence.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry