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Addgene

pLV floxed hMyoD-IRES-dsRed
(Plasmid #66632)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66632 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Total vector size (bp) 11500
  • Vector type
    Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human MyoD IRES dsRed
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2200
  • Entrez Gene
    MYOD1 (a.k.a. CMYP17, MYF3, MYOD, MYODRIF, PUM, bHLHc1)
  • Promoter hUbC

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGTACAGTGCAGGGGAAAGA
  • 3′ sequencing primer GGCATTAAAGCAGCGTATCC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV floxed hMyoD-IRES-dsRed was a gift from Charles Gersbach (Addgene plasmid # 66632 ; http://n2t.net/addgene:66632 ; RRID:Addgene_66632)
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