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Addgene

pECFP-Sec23B
(Plasmid #66615)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec23B
  • Species
    M. musculus (mouse)
  • Mutation
    A2S, T3S, Y4S, L5F
  • GenBank ID
    AAH05464.1
  • Tag / Fusion Protein
    • CFP (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor states that mutations do not not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECFP-Sec23B was a gift from David Stephens (Addgene plasmid # 66615 ; http://n2t.net/addgene:66615 ; RRID:Addgene_66615)

  • For your References section:

    Characterization of human Sec16B: indications of specialized, non-redundant functions. Budnik A, Heesom KJ, Stephens DJ. Sci Rep. 2011;1:77. doi: 10.1038/srep00077. Epub 2011 Aug 30. 10.1038/srep00077 PubMed 22355596
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