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PurposeCFP fusion of Sec31A
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66612 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepECFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec31A
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SpeciesH. sapiens (human)
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GenBank IDNM_014933.3
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Entrez GeneSEC31A (a.k.a. ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1)
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Tag
/ Fusion Protein
- ECFP (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pECFP-Sec31A was a gift from David Stephens (Addgene plasmid # 66612 ; http://n2t.net/addgene:66612 ; RRID:Addgene_66612) -
For your References section:
COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites. Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP. J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85. PubMed 10825291