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Addgene

pECFP-Sec31A
(Plasmid #66612)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66612 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec31A
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_014933.3
  • Entrez Gene
    SEC31A (a.k.a. ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1)
  • Tag / Fusion Protein
    • ECFP (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECFP-Sec31A was a gift from David Stephens (Addgene plasmid # 66612 ; http://n2t.net/addgene:66612 ; RRID:Addgene_66612)
  • For your References section:

    COPI-coated ER-to-Golgi transport complexes segregate from COPII in close proximity to ER exit sites. Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP. J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85. PubMed 10825291