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pSEW-Venus-Sec16A-deltaN
(Plasmid #66605)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66605 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVenus-C1
  • Backbone manufacturer
    Clontech, then modified
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec16A
  • Alt name
    KIAA0310
  • Species
    H. sapiens (human)
  • Mutation
    encodes amino acids 1107-1217 of human Sec16A
  • Entrez Gene
    SEC16A (a.k.a. KIAA0310, SEC16L, p250)
  • Tag / Fusion Protein
    • Venus (EYFP derivative) (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEW-Venus-Sec16A-deltaN was a gift from David Stephens (Addgene plasmid # 66605 ; http://n2t.net/addgene:66605 ; RRID:Addgene_66605)

  • For your References section:

    Sec16A defines the site for vesicle budding from the endoplasmic reticulum on exit from mitosis. Hughes H, Stephens DJ. J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. Epub 2010 Nov 2. 10.1242/jcs.076000 PubMed 21045114
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