pLVXPuro-epsilon-COP-GFP
(Plasmid
#66604)
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PurposeExpresses epsilon COP as a GFP fusion
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLVX-Puro
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Backbone manufacturerClontech
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameepsilon COP
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Alt nameeCOP
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SpeciesB. taurus (bovine)
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GenBank IDNP_788846.1
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Entrez GeneCOPE
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Epsilon COP GFP originally described in
Presley, J. F., Ward, T. H., Pfeifer, A. C., Siggia, E. D., Phair, R. D. and Lippincott-Schwartz, J. (2002) Dissection of COPI and Arf1 dynamics in vivo and role in Golgi membrane transport. Nature 417, 187-193
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVXPuro-epsilon-COP-GFP was a gift from David Stephens (Addgene plasmid # 66604 ; http://n2t.net/addgene:66604 ; RRID:Addgene_66604) -
For your References section:
Phosphorylation and membrane dissociation of the ARF exchange factor GBF1 in mitosis. Morohashi Y, Balklava Z, Ball M, Hughes H, Lowe M. Biochem J. 2010 Apr 14;427(3):401-12. doi: 10.1042/BJ20091681. 10.1042/BJ20091681 PubMed 20175751