pECFP-N2-COL1A1
(Plasmid
#66603)
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PurposeExpresses procollagen 1 alpha 1 as a CFP fusion
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 66603 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepECFP-N2
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsDifficult to grow and prep without apparent loss of some of insert.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCOL1A1
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Alt nameProcollagen 1alpha1
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SpeciesH. sapiens (human)
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GenBank IDNM_000088.3
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Entrez GeneCOL1A1 (a.k.a. CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Insert contains T1434S compared to all reference sequences. Depositor states that this mutation does not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pECFP-N2-COL1A1 was a gift from David Stephens (Addgene plasmid # 66603 ; http://n2t.net/addgene:66603 ; RRID:Addgene_66603) -
For your References section:
Imaging of procollagen transport reveals COPI-dependent cargo sorting during ER-to-Golgi transport in mammalian cells. Stephens DJ, Pepperkok R. J Cell Sci. 2002 Mar 15;115(Pt 6):1149-60. PubMed 11884515