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pLVX-Puro-mRuby-WDR34
(Plasmid #66599)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX-Puro
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WDR34
  • Alt name
    WD34
  • Alt name
    FAP133
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_052844.3
  • Entrez Gene
    WDR34 (a.k.a. DIC5, FAP133, SRTD11, bA216B9.3)
  • Tag / Fusion Protein
    • mRuby (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mRuby was synthesized to our requirements by Eurofins MWG

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The cDNA sequence encoding mRuby (Kredel et al., 2009) underwent codon usage
adaptation before gene synthesis (MWG Eurofins) and cloning into pBluescript II
SK(+). mRuby was excised with SalI-EcoRI for insertion into pLVX-Puro (Clontech)
to generate pLVX-Puro–mRuby. A cDNA encoding human Sec23A was amplified
by PCR, gel purified and subcloned via pGEM-T vector (excised with ApaI-XbaI)
and cloned into pLVX-Puro–mRuby.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-Puro-mRuby-WDR34 was a gift from David Stephens (Addgene plasmid # 66599 ; http://n2t.net/addgene:66599 ; RRID:Addgene_66599)

  • For your References section:

    Subunit composition of the human cytoplasmic dynein-2 complex. Asante D, Stevenson NL, Stephens DJ. J Cell Sci. 2014 Nov 1;127(21):4774-87. doi: 10.1242/jcs.159038. Epub 2014 Sep 9. 10.1242/jcs.159038 PubMed 25205765
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