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PurposeExpresses Sec23B as a fusion with mGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 66598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX-Puro
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Backbone manufacturerClontech
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec23B
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SpeciesH. sapiens (human)
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Entrez GeneSEC23B (a.k.a. CDA-II, CDAII, CDAN2, CWS7, HEMPAS, hSec23B)
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Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymGFP was synthesized to our requirements by Eurofins MWG
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVXPuro-mGFP-Sec23B was a gift from David Stephens (Addgene plasmid # 66598 ; http://n2t.net/addgene:66598 ; RRID:Addgene_66598) -
For your References section:
Sec16A defines the site for vesicle budding from the endoplasmic reticulum on exit from mitosis. Hughes H, Stephens DJ. J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8. Epub 2010 Nov 2. 10.1242/jcs.076000 PubMed 21045114