pCIneo-lambdaN-HA-HsEDC4
(Plasmid #66597)

• Purpose: Expresses lambdaN-HA-tagged EDC4 in mammalian cells
• Depositing Lab: Elisa Izaurralde
• Publication: Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6.

Backbone

• Vector backbone: pCIneo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5554
• Total vector size (bp): 9760
• Modifications to backbone: introduced a lambdaN-HA-tag
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EDC4
• Alt name: Ge-1
• Alt name: Hedl5
• Alt name: RCD8
• Species: H. sapiens (human)
• Insert Size (bp): 4206
• GenBank ID: NM_014329.4
• Entrez Gene: EDC4 (a.k.a. GE1, Ge-1, HEDL5, HEDLS, RCD-8, RCD8)
• Promoter: CMV
• Tag / Fusion Protein:
  • lambdaN-HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-forw

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry