pLNHA-C1-HsXRN1
(Plasmid #66596)

• Purpose: expresses lambdaN-HA-tagged XRN1 in mammalian cells
• Depositing Lab: Elisa Izaurralde
• Publication: Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 10.1038/nsmb.2413. Epub 2012 Nov 11.

Backbone

• Vector backbone: pLNHA-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4174
• Total vector size (bp): 9295
• Modifications to backbone: Exchanged the EGFP tag from pEGFP-C1 by a lambdaN-HA-tag
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: XRN1
• Alt name: SEP1
• Species: H. sapiens (human)
• Insert Size (bp): 5121
• GenBank ID: NM_019001
• Entrez Gene: XRN1 (a.k.a. SEP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • lambdaN-HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV for

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry