ins:CFP-NTR
(Plasmid #66593)

• Purpose: Specific and conditional cell ablation of beta cells in the zebrafish pancreas
• Depositing Lab: Didier Stainier
• Publication: Curado et al Dev Dyn. 2007 Apr . 236(4):1025-35.

Backbone

• Vector backbone: I-Sce I element containing vector
• Vector type: zebrafish expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Insulin promotor driving CFP-nitroreductase
• Alt name: ins:CFP-Eco.NfsB
• Species: D. rerio (zebrafish); Aequorea victoria, E.coli
• Insert Size (bp): 3000
• Promoter: Zebrafish Insulin promoter (approximately 1.2kb)
• Tag / Fusion Protein:
  • CFP fused with N-terminus of nitroreductase (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GCTATTCGTCCCAAAACATCTCCAC
• 3′ sequencing primer: TAGCATCACAAATTTCACAAATAAAGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry