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pAc5.1B-EGFP-DmDis3
(Plasmid #66592)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6051
  • Total vector size (bp) 9000
  • Modifications to backbone
    N-terminal EGFP-tag
  • Vector type
    Drosophila Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DIS3
  • Alt name
    CG6413
  • Alt name
    Rrp44
  • Species
    D. melanogaster (fly)
  • GenBank ID
    NM_142989
  • Entrez Gene
    Dis3 (a.k.a. Dmel_CG6413, CG6413, Dmel\CG6413, Rrp44, dDis3, dRrp44, dis3, taz)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HIndIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-forw
  • 3′ sequencing primer Bgh-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmDis3 was a gift from Elisa Izaurralde (Addgene plasmid # 66592 ; http://n2t.net/addgene:66592 ; RRID:Addgene_66592)
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