Skip to main content
Addgene

pAc5.1B-EGFP-DmMTR-4
(Plasmid #66591)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6057
  • Total vector size (bp) 9225
  • Modifications to backbone
    N-terminal EGFP-tag
  • Vector type
    Drosophila Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MTR-4
  • Alt name
    lethal (2)35Df
  • Alt name
    CG4152
  • Species
    D. melanogaster (fly)
  • GenBank ID
    NM_080190
  • Entrez Gene
    l(2)35Df (a.k.a. Dmel_CG4152, BG:DS09217.2, BcDNA:GH07290, CG4152, Dmel\CG4152, Mtr4, br44, cg4152, l(2)br44, l(2)k14422, l(2)k14423, l.2.35Df, lethal(2)35Df)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-forw
  • 3′ sequencing primer Bgh-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmMTR-4 was a gift from Elisa Izaurralde (Addgene plasmid # 66591 ; http://n2t.net/addgene:66591 ; RRID:Addgene_66591)
Related items:
Commonly requested with: