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Addgene

pCyc-Bem1
(Plasmid #66573)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66573 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Yeast integrating, TRP
  • Backbone size w/o insert (bp) 6793
  • Total vector size (bp) 8449
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bem1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1656
  • Mutation
    F99I*(see below)
  • Promoter pCyc1

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note: Addgene's quality control sequencing finds an F99I amino acid residue substitution in the Bem1 insert. The effect of this residue change is unknown.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCyc-Bem1 was a gift from Orion Weiner (Addgene plasmid # 66573 ; http://n2t.net/addgene:66573 ; RRID:Addgene_66573)

  • For your References section:

    Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. Jost AP, Weiner OD. ACS Synth Biol. 2015 Jun 2. 10.1021/acssynbio.5b00053 PubMed 26035630
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