PhyB-mCherry-Tom7
(Plasmid #66571)

• Purpose: Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae
• Depositing Lab: Orion Weiner
• Publication: Jost et al ACS Synth Biol. 2015 Jun 2.

Backbone

• Vector backbone: pRS305
• Total vector size (bp): 12448
• Vector type: Yeast Expression
• Selectable markers: LEU2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PhyB
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2724
• Mutation: PhyB portion contains aa's 1-908
• Entrez Gene: PHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
• Promoter: pADH1
• Tags / Fusion Proteins:
  • mCherry (C terminal on insert)
  • Tom7 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: mCherry F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Jared Toettcher and Xiaojing Yang, UCSF

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry