CelTag Plasmid
(Plasmid #66562)

• Purpose: Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.
• Depositing Lab: David Engelke
• Publication: Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.025106. doi: 10.1534/g3.115.025106.

Backbone

• Vector backbone: pGEM®-T Easy Vector
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015
• Total vector size (bp): 5573
• Modifications to backbone: Addition of CelTag and cloNat resistance.
• Vector type: Bacterial Expression
• Selectable markers: Nourseothricin (cloNat)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Ampicillin selection is sufficient for plasmid preparation. Nourseothricin selection is used to confirm insertion of the CelTag
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CelTag
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2558

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The 13x myc epitope repeat and cloNat resistance gene were obtained from the pFA6a-13myc-natMX6 plasmid (gift from Dr. Antony Carr at University of Sussex), and the CBM3 was obtained from the pCIG plasmid (gift from Dr. Jiong Hong at Virginia Polytechnic Institute and State University)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry