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pETM6-E12-vioABECD
(Plasmid #66537)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66537 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM6
  • Backbone size w/o insert (bp) 5155
  • Total vector size (bp) 13599
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VioA
  • Species
    Pseudoalteromonas luteoviolacea
  • Insert Size (bp)
    1290
  • Promoter Mutant T7 Promoter - H10

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGTTAAATTTAGAGCATTCAG
  • 3′ sequencing primer TCAAAGGTATACTACTTCTTTCAC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    VioB
  • Species
    Pseudoalteromonas luteoviolacea
  • Insert Size (bp)
    3030
  • Promoter Mutant T7 Promoter - G6

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGAGTGTTTTAGATTTTCCTCG
  • 3′ sequencing primer CTAACCTTCCTTTGAAAG
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    VioE
  • Species
    Pseudoalteromonas luteoviolacea
  • Insert Size (bp)
    606
  • Promoter Mutant T7 Promoter - G6

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGGAATTACGTAAAGTAGATAGAGTTCC
  • 3′ sequencing primer TCAATTCCTATGAGAGAGAC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    VioC
  • Species
    Pseudoalteromonas luteoviolacea
  • Insert Size (bp)
    1290
  • Promoter Mutant T7 Promoter - H10

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGAGTAAAATAATTATTGTTGGTGGTG
  • 3′ sequencing primer TTAATTCATTCTCCCTATTTTGTAC
    • (Common Sequencing Primers)

Gene/Insert 5

  • Gene/Insert name
    VioD
  • Species
    Pseudoalteromonas luteoviolacea
  • Insert Size (bp)
    1122
  • Promoter Mutant T7 Promoter - G6

Cloning Information for Gene/Insert 5

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATGAACATTTTAGTGATCGGG
  • 3′ sequencing primer GAGTTAACGTTGCAGCGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETM6-E12-vioABECD was a gift from Mattheos Koffas (Addgene plasmid # 66537 ; http://n2t.net/addgene:66537 ; RRID:Addgene_66537)

  • For your References section:

    ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Jones JA, Vernacchio VR, Lachance DM, Lebovich M, Fu L, Shirke AN, Schultz VL, Cress B, Linhardt RJ, Koffas MA. Sci Rep. 2015 Jun 11;5:11301. doi: 10.1038/srep11301. 10.1038/srep11301 PubMed 26062452
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