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pAc5.1B-EGFP-DmTRF4-2
(Plasmid #66204)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 66204 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAc5.1B-EGFP
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6057
  • Total vector size (bp) 7281
  • Modifications to backbone
    N-terminal EGFP-tag
  • Vector type
    Drosophila Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TRF4-2
  • Alt name
    CG17462
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1224
  • GenBank ID
    NM_143056
  • Entrez Gene
    Trf4-2 (a.k.a. Dmel_CG17462, AAF56369, CG17462, DmTRF4-2, Dmel\CG17462)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-forw
  • 3′ sequencing primer Bgh-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc5.1B-EGFP-DmTRF4-2 was a gift from Elisa Izaurralde (Addgene plasmid # 66204 ; http://n2t.net/addgene:66204 ; RRID:Addgene_66204)
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